Introgression of clubroot resistant gene into Brassica oleracea L. from Brassica rapa based on homoeologous exchange.

ABSTRACT: Clubroot is a soil-borne disease in cabbage (Brassica oleracea L. var. capitata L.) caused by Plasmodiophora brassicae, which poses a great threat to cabbage production. However, clubroot resistance (CR) genes in Brassica rapa could be introduced into the cabbage via breeding to make it clubroot resistant. In this study, CR genes from B. rapa were introduced into the cabbage genome and the mechanism of gene introgression was explored. Two methods were used to create CR materials: (i) The fertility of CR Ogura CMS cabbage germplasms containing CRa was restored by using an Ogura CMS restorer. After cytoplasmic replacement and microspore culture, CRa-positive microspore individuals were obtained. (ii) Distant hybridization was performed between

SUBMITTER: Zhu M 

PROVIDER: S-EPMC10167419 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature