Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination.

ABSTRACT: HSV glycoproteins play important roles in the viral life cycle, particularly viral cell entry. Here we describe the protocol for expression, purification, and crystallization of full-length HSV-1 glycoprotein B. The protocol provides a framework for incorporating transmembrane domain-stabilizing amphipols into the crystallization setup and can be adapted to isolate other complete HSV glycoproteins.

SUBMITTER: Cooper RS 

PROVIDER: S-EPMC10167678 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature