Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain.

ABSTRACT: Proteinaceous fibrillar inclusions are key pathological hallmarks of multiple neurodegenerative diseases. In the early stages of Alzheimer's disease (AD), amyloid-beta peptides form protofibrils in the extracellular space, which act as seeds that gradually grow and mature into large amyloid plaques. Despite this basic understanding, current knowledge of the amyloid fibril structure, composition, and deposition patterns in the brain is limited. One major barrier has been the inability to isolate highly purified amyloid fibrils from brain extracts. Affinity purification and laser capture microdissection-based approaches have been previously used to isolate amyloids but are limited by the small quantity of material that can be recovered. This novel, robust protocol describes the biochemical p

SUBMITTER: Upadhyay A 

PROVIDER: S-EPMC10167712 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature