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Isolation and characterization of single domain antibodies from banded houndshark (Triakis scyllium) targeting SARS-CoV-2 spike RBD protein.

ABSTRACT: The COVID-19 pandemic has significantly impacted human health for three years. To mitigate the spread of SARS-CoV-2, the development of neutralizing antibodies has been accelerated, including the exploration of alternative antibody formats such as single-domain antibodies. In this study, we identified variable new antigen receptors (VNARs) specific for the receptor binding domain (RBD) of SARS-CoV-2 by immunizing a banded houndshark (Triakis scyllium) with recombinant wild-type RBD. Notably, the CoV2NAR-1 clone showed high binding affinities in the nanomolar range to various RBDs and demonstrated neutralizing activity against SARS-CoV-2 pseudoviruses. These results highlight the potential of the banded houndshark as an animal model for the development of VNAR-based therapeutics or diagnostics against future pandemics.

SUBMITTER: Kim WS 

PROVIDER: S-EPMC10167778 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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Isolation and characterization of single domain antibodies from banded houndshark (Triakis scyllium) targeting SARS-CoV-2 spike RBD protein.

Kim Woo Sung WS   Chae Hee Do HD   Jung Inji I   Lee Won-Kyu WK   Lee Woo Jun WJ   Lee Jisun J   Gong Yejin Y   Lee Dohyun D   Kim Byeong-Won BW   Kim Jin-Koo JK   Hwang Jaehyeon J   Kweon Dae-Hyuk DH   Jung Sang Taek ST   Na Jung-Hyun JH  

Fish & shellfish immunology 20230509

The COVID-19 pandemic has significantly impacted human health for three years. To mitigate the spread of SARS-CoV-2, the development of neutralizing antibodies has been accelerated, including the exploration of alternative antibody formats such as single-domain antibodies. In this study, we identified variable new antigen receptors (VNARs) specific for the receptor binding domain (RBD) of SARS-CoV-2 by immunizing a banded houndshark (Triakis scyllium) with recombinant wild-type RBD. Notably, the  ...[more]

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