Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.

ABSTRACT: Sugars play important roles in numerous biological processes, from providing energy to modifying proteins to alter their function. Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification. Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further. Sugars, however, are difficult analytes to probe due to their isomeric nature. In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes. The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross secti

SUBMITTER: Schorr HC 

PROVIDER: S-EPMC10167912 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature