DNA nanotechnology in the undergraduate laboratory: Electrophoretic analysis of DNA nanostructure biostability.

ABSTRACT: The field of DNA nanotechnology has grown rapidly in the last decade and has expanded to multiple laboratories. While lectures in DNA nanotechnology have been introduced in some institutions, laboratory components at the undergraduate level are still lacking. Undergraduate students predominantly learn about DNA nanotechnology through their involvement as interns in research laboratories. The DNA nanostructure biostability analysis experiment presented here can be used as a hands-on introductory laboratory exercise for discussing concepts in DNA nanotechnology in an undergraduate setting. This experiment discusses biostability, gel electrophoresis and quantitative analysis of nuclease degradation of a model DNA nanostructure, the paranemic crossover (PX) DNA motif. The experiment can be per

SUBMITTER: Chandrasekaran AR 

PROVIDER: S-EPMC10168010 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature