Dataset Information
ANDA: An open-source tool for automated image analysis of neuronal differentiation.
Ontology highlight
ABSTRACT: Background
Imaging of in vitro neuronal differentiation and measurements of cell morphologies has led to novel insights into neuronal development. Live-cell imaging techniques and large datasets of images has increased the demand for automated pipelines for quantitative analysis of neuronal morphological metrics.Results
We present ANDA, an analysis workflow for quantification of various aspects of neuronal morphology from high-throughput live-cell imaging screens. This tool automates the analysis of neuronal cell numbers, neurite lengths and neurite attachment points. We used rat, chicken and human in vitro models for neuronal differentiation and have demonstrated the accuracy, versatility, and efficiency of the tool.Conclusions
ANDA is an open-source tool that is easy to use and capable of automated processing from time-course measurements of neuronal cells. The strength of this pipeline is the capability to analyse high-throughput imaging screens.
SUBMITTER: Wæhler HA
PROVIDER: S-EPMC10168306 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
Publications
ANDA: An open-source tool for automated image analysis of neuronal differentiation.
bioRxiv : the preprint server for biology 20230428
<h4>Background</h4>Imaging of <i>in vitro</i> neuronal differentiation and measurements of cell morphologies has led to novel insights into neuronal development. Live-cell imaging techniques and large datasets of images has increased the demand for automated pipelines for quantitative analysis of neuronal morphological metrics.<h4>Results</h4>We present ANDA, an analysis workflow for quantification of various aspects of neuronal morphology from high-throughput live-cell imaging screens. This too ...[more]