Local Monomer Levels and Established Filaments Potentiate Non-Muscle Myosin 2 Assembly.

ABSTRACT: The ability to dynamically assemble contractile networks is required throughout cell physiology, yet the biophysical mechanisms regulating non-muscle myosin 2 filament assembly in living cells are lacking. Here we use a suite of dynamic, quantitative imaging approaches to identify deterministic factors that drive myosin filament appearance and amplification. We find that actin dynamics regulate myosin assembly, but that the actin architecture plays a minimal direct role. Instead, remodeling of actin networks modulates the local myosin monomer levels and facilitates assembly through myosin:myosin driven interactions. Using optogenetically controlled myosin, we demonstrate that locally concentrating myosin is sufficient to both form filaments and jump-start filament amplification and partiti

SUBMITTER: Quintanilla MA 

PROVIDER: S-EPMC10168331 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature