CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions.

ABSTRACT: The sheer complexity of the brain has complicated our ability to understand the cellular and molecular mechanisms underlying its function in health and disease. Genome-wide association studies have uncovered genetic variants associated with specific neurological phenotypes and diseases. In addition, single-cell transcriptomics have provided molecular descriptions of specific brain cell types and the changes they undergo during disease. Although these approaches provide a giant leap forward towards understanding how genetic variation can lead to functional changes in the brain, they do not establish molecular mechanisms. To address this need, we developed a 3D co-culture system termed iAssembloids (induced multi-lineage assembloids) that enables the rapid generation of homogenous neuron-gli

SUBMITTER: Li E 

PROVIDER: S-EPMC10168378 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature