Encystation stimuli sensing mediated by adenylate cyclase AC2-dependent cAMP signaling in Giardia.

ABSTRACT: Protozoan parasites use cAMP signaling to precisely regulate the place and time of developmental differentiation, yet it is unclear how this signaling is initiated. Encystation of the intestinal parasite Giardia lamblia can be activated by multiple stimuli, which we hypothesize result in a common physiological change. We demonstrate that bile alters plasma membrane fluidity by reducing cholesterol-rich lipid microdomains, while alkaline pH enhances bile function. Through depletion of the cAMP producing enzyme Adenylate Cyclase 2 (AC2) and the use of a newly developed Giardia-specific cAMP sensor, we show that AC2 is necessary for encystation stimuli-induced cAMP upregulation and activation of downstream signaling. Conversely, over expression of AC2 or exogenous cAMP were suff

SUBMITTER: Shih HW 

PROVIDER: S-EPMC10168462 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature