Single-cell profiling reveals immune aberrations in progressive idiopathic pulmonary fibrosis.

ABSTRACT:

Rationale

Changes in peripheral blood cell populations have been observed but not detailed at single-cell resolution in idiopathic pulmonary fibrosis (IPF).

Objectives

To provide an atlas of the changes in the peripheral immune system in stable and progressive IPF.

Methods

Peripheral blood mononuclear cells (PBMCs) from IPF patients and controls were profiled using 10x Chromium 5' single-cell RNA sequencing (scRNA-seq). Flow cytometry was used for validation. Protein concentrations of Regulatory T-cells (Tregs) and Monocytes chemoattractants were measured in plasma and lung homogenates from patients and controls.

Measurements and main results

Thirty-eight PBMC samples from 25 patients with IPF and 13 matched controls yielded 149,564 cells that segregated into 2

SUBMITTER: Unterman A 

PROVIDER: S-EPMC10168511 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature