Double emulsions as a high-throughput enrichment and isolation platform for slower-growing microbes.

ABSTRACT: Our understanding of in situ microbial physiology is primarily based on physiological characterization of fast-growing and readily-isolatable microbes. Microbial enrichments to obtain novel isolates with slower growth rates or physiologies adapted to low nutrient environments are plagued by intrinsic biases for fastest-growing species when using standard laboratory isolation protocols. New cultivation tools to minimize these biases and enrich for less well-studied taxa are needed. In this study, we developed a high-throughput bacterial enrichment platform based on single cell encapsulation and growth within double emulsions (GrowMiDE). We showed that GrowMiDE can cultivate many different microorganisms and enrich for underrepresented taxa that are never observed in traditional batch enrich

SUBMITTER: McCully AL 

PROVIDER: S-EPMC10169782 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature