Influence of bacterial and alveolar cell co-culture on microbial VOC production using HS-GC/MS.

ABSTRACT: Volatile organic compounds (VOCs) found in exhaled breath continue to garner interest as an alternative diagnostic tool in pulmonary infections yet, their clinical integration remains a challenge with difficulties in translating identified biomarkers. Alterations in bacterial metabolism secondary to host nutritional availability may explain this but is often inadequately modelled in vitro. The influence of more clinically relevant nutrients on VOC production for two common respiratory pathogens was investigated. VOCs from Staphylococcus aureus (S.aureus) and Pseudomonas aeruginosa (P.aeruginosa) cultured with and without human alveolar A549 epithelial cells were analyzed using headspace extraction coupled with gas chromatography-mass spectrometry. Untarge

SUBMITTER: Fenn D 

PROVIDER: S-EPMC10169821 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature