Molecular characterization of the Indian isolate (Ka-To) of tomato spotted wilt virus (TSWV) infecting tomato (Solanum lycopersicum L.).

ABSTRACT: Tomato spotted wilt virus (TSWV) infecting tomato has been identified as an emerging constraint for tomato cultivation in the southern Indian states of Karnataka and Tamil Nadu. Infection of TSWV produces circular necrotic ring spots on leaves, stem and floral necrosis and necrotic ringspots on fruits of tomato. In this study, we describe the characterization of TSWV isolate (Ka-To) infecting tomato from India based on biological, serological and molecular assay. Pathogenicity of TSWV (Ka-To) isolate was established by mechanical inoculation of sap from infected leaves on tomato, cowpea and datura which expressed necrotic or chlorotic local lesions. Samples were tested positive in the serological assay performed with TSWV-specific immunostrips. Further, reverse transcription polymerase cha

SUBMITTER: Aravintharaj R 

PROVIDER: S-EPMC10169989 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature