Overexpression of miR-32 in Chinese hamster ovary cells increases production of Fc-fusion protein.

ABSTRACT: The demand for industrial genetically modified host cells were increased with the growth of the biopharmaceutical market. Numerous studies on improving host cell productivity have shown that altering host cell growth and viability through genetic engineering can increase recombinant protein production. During the last decades, it was demonstrated that overexpression or downregulation of some microRNAs in Chinese Hamster Ovary (CHO) cells as the host cell in biopharmaceutical manufacturing, can improve their productivity. The selection of microRNA targets has been based on their previously identified role in human cancers. MicroRNA-32 (miR-32), which is conserved between humans and hamsters (Crisetulus griseus), was shown to play a role in the regulation of cell proliferation and apoptosis

SUBMITTER: Bazaz M 

PROVIDER: S-EPMC10170017 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature