Identification and validation of the reference genes in the echiuran worm Urechis unicinctus based on transcriptome data.

ABSTRACT:

Background

Real-time quantitative PCR (RT-qPCR) is a crucial and widely used method for gene expression analysis. Selecting suitable reference genes is extremely important for the accuracy of RT-qPCR results. Commonly used reference genes are not always stable in various organisms or under different environmental conditions. With the increasing application of high-throughput sequencing, transcriptome analysis has become an effective method for identifying novel stable reference genes.

Results

In this study, we identified candidate reference genes based on transcriptome data covering embryos and larvae of early development, normal adult tissues, and the hindgut under sulfide stress using the coefficient of variation (CV) method in the echiuran Urechis unicinctus, resulting in

SUBMITTER: Chen J 

PROVIDER: S-EPMC10170059 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature