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Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase.

ABSTRACT: L-DOPA is an amino acid that is used as a treatment for Parkinson's disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-overproducing strains is described, which were developed based on mutations in a DNA binding protein, TyrR, which controls the induction of tpl gene expression.

SUBMITTER: Kumagai H

PROVIDER: S-EPMC10170061 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase.

Kumagai Hidehiko H Katayama Takane T Koyanagi Takashi T Suzuki Hideyuki H

Proceedings of the Japan Academy. Series B, Physical and biological sciences 20230101 3

L-DOPA is an amino acid that is used as a treatment for Parkinson's disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-overproducing strains is described, which were develo ...[more]

PMID: 36908174

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