Decellularized extracellular matrix as scaffold for cancer organoid cultures of colorectal peritoneal metastases.

ABSTRACT: Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC metastases to the peritoneum. The mechanisms underlying the interactions between metastatic cells and the ECM, however, remain poorly understood, and the number of in vitro models available for the study of the peritoneal metastatic process is limited. Here, we show that decellularized ECM of the peritoneal cavity allows the growth of organoids obtained from PM, favoring the development of three-dimensional (3D) nodules that maintain the characteristics of in vivo PM. Organoids preferentially grow on scaffolds obtained from neoplastic peritoneum, which are characterized by greater stiffness than normal scaffolds.

SUBMITTER: Varinelli L 

PROVIDER: S-EPMC10170410 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature