CRISPR-Cas9 knockout screen identifies novel treatment targets in childhood high-grade glioma.

ABSTRACT:

Background

Brain tumours are the leading cause of cancer-related death in children, and there is no effective treatment. A growing body of evidence points to deregulated epigenetics as a tumour driver, particularly in paediatric cancers as they have relatively few genomic alterations, and key driver mutations have been identified in histone 3 (H3). Cancer stem cells (CSC) are implicated in tumour development, relapse and therapy resistance and thus particularly important to target. We therefore aimed to identify novel epigenetic treatment targets in CSC derived from H3-mutated high-grade glioma (HGG) through a CRISPR-Cas9 knockout screen.

Results

The knockout screen identified more than 100 novel genes essential for the growth of CSC derived from paediatric HGG with H3K27M m

SUBMITTER: Wenger A 

PROVIDER: S-EPMC10170782 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature