Genome-wide analysis of RNA-binding proteins co-expression with alternative splicing events in mitral valve prolapse.

ABSTRACT:

Objectives

We investigated the role and molecular mechanisms of RNA-binding proteins (RBPs) and their regulated alternative splicing events (RASEs) in the pathogenesis of mitral valve prolapse (MVP).

Methods

For RNA extraction, we obtained peripheral blood mononuclear cells (PBMCs) from five patients with MVP, with or without chordae tendineae rupture, and five healthy individuals. High-throughput sequencing was used for RNA sequencing (RNA-seq). Differentially expressed genes (DEGs) analysis, alternative splicing (AS) analysis, functional enrichment analysis, co-expression of RBPs, and alternative splicing events (ASEs) analysis were conducted.

Results

The MVP patients exhibited 306 up-regulated genes and 198 down-regulated genes. All down- and up-regulated genes wer

SUBMITTER: Zhao M 

PROVIDER: S-EPMC10171460 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature