A validated protocol to UV-inactivate SARS-CoV-2 and herpesvirus-infected cells.

ABSTRACT: Downstream analysis of virus-infected cell samples, such as reverse transcription polymerase chain reaction (RT PCR) or mass spectrometry, often needs to be performed at lower biosafety levels than their actual cultivation, and thus the samples require inactivation before they can be transferred. Common inactivation methods involve chemical crosslinking with formaldehyde or denaturing samples with strong detergents, such as sodium dodecyl sulfate. However, these protocols destroy the protein quaternary structure and prevent the analysis of protein complexes, albeit through different chemical mechanisms. This often leads to studies being performed in over-expression or surrogate model systems. To address this problem, we generated a protocol that achieves the inactivation of infected cells

SUBMITTER: Soh TK 

PROVIDER: S-EPMC10171616 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature