Extracellular vesicles from human plasma for biomarkers discovery: Impact of anticoagulants and isolation techniques.

ABSTRACT: Extracellular vesicles (EVs) isolated from plasma are increasingly recognized as promising circulating biomarkers for disease discovery and progression, as well as for therapeutic drug delivery. The scientific community underlined the necessity of standard operative procedures for the isolation and storage of the EVs to ensure robust results. The understanding of the impact of the pre-analytical variables is still limited and some considerations about plasma anticoagulants and isolation methods are necessary. Therefore, we performed a comparison study between EVs isolated by ultracentrifugation and by affinity substrate separation from plasma EDTA and sodium citrate. The EVs were characterized by Nano Tracking Analysis, Western Blot, cytofluorimetric analysis of surface markers, and lipido

SUBMITTER: Bettio V 

PROVIDER: S-EPMC10171685 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature