3D-printed microgels supplemented with dentin matrix molecules as a novel biomaterial for direct pulp capping.

ABSTRACT:

Objectives

To develop a 3D-printed, microparticulate hydrogel supplemented with dentin matrix molecules (DMM) as a novel regenerative strategy for dental pulp capping.

Materials and methods

Gelatin methacryloyl microgels (7% w/v) mixed with varying concentrations of DMM were printed using a digital light projection 3D printer and lyophilized for 2 days. The release profile of the DMM-loaded microgels was measured using a bicinchoninic acid assay. Next, dental pulp exposure defects were created in maxillary first molars of Wistar rats. The exposures were randomly capped with (1) inert material - negative control, (2) microgels, (3) microgels + DMM 500 µg/ml, (4) microgels + DMM 1000 µg/ml, (5) microgels + platelet-derived growth factor (PDGF 10 ng/ml), or (6) MTA (n = 15/grou

SUBMITTER: Cunha D 

PROVIDER: S-EPMC10171721 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature