Bioengineered perfused human brain microvascular networks enhance neural progenitor cell survival, neurogenesis, and maturation.

ABSTRACT: Neural progenitor cells (NPCs) have the capability to self-renew and differentiate into neurons and glial cells. In the adult brain, NPCs are found near brain microvascular networks (BMVNs) in specialized microenvironments called the neurovascular niche (NVN). Although several in vitro NVN models have been previously reported, most do not properly recapitulate the intimate cellular interactions between NPCs and perfused brain microvessels. Here, we developed perfused BMVNs composed of primary human brain endothelial cells, pericytes, and astrocytes within microfluidic devices. When induced pluripotent stem cell-derived NPCs were introduced into BMVNs, we found that NPC survival, neurogenesis, and maturation were enhanced. The application of flow during BMVN coculture was also beneficial fo

SUBMITTER: Winkelman MA 

PROVIDER: S-EPMC10171804 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature