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High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development.

ABSTRACT: Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.

SUBMITTER: Bevilacqua C

PROVIDER: S-EPMC10172129 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development.

Bevilacqua Carlo C Gomez Juan Manuel JM Fiuza Ulla-Maj UM Chan Chii Jou CJ Wang Ling L Hambura Sebastian S Eguren Manuel M Ellenberg Jan J Diz-Muñoz Alba A Leptin Maria M Prevedel Robert R

Nature methods 20230330 5

Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combinatio ...[more]

PMID: 36997817

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