A light-gated cation channel with high reactivity to weak light.

ABSTRACT: The cryptophyte algae, Guillardia theta, possesses 46 genes that are homologous to microbial rhodopsins. Five of them are functionally light-gated cation channelrhodopsins (GtCCR1-5) that are phylogenetically distinct from chlorophyte channelrhodopsins (ChRs) such as ChR2 from Chlamydomonas reinhardtii. In this study, we report the ion channel properties of these five CCRs and compared them with ChR2 and other ChRs widely used in optogenetics. We revealed that light sensitivity varied among GtCCR1-5, in which GtCCR1-3 exhibited an apparent EC₅₀ of 0.21-1.16 mW/mm², similar to that of ChR2, whereas GtCCR4 and GtCCR5 possess two EC50s, one of which is significantly small (0.025 and 0.032 mW/mm²). GtCCR4 is able to trigger action potentials in high temporal re

SUBMITTER: Hososhima S 

PROVIDER: S-EPMC10172181 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature