Conservation and variation in the region of the Theileria parva p104 antigen coding gene used for PCR surveillance of the parasite.

ABSTRACT: The range of the protozoan parasite Theileria parva, which causes East Coast fever in cattle, has been expanding to countries where it has not previously been detected, as a result of cross-border domestic cattle movement. Countries where T. parva has not previously been observed until recently include Cameroon and South Sudan. This raises the issue of the conservation of the p104 antigen gene, on which the nested PCR assay that is widely used for T. parva surveillance in the blood of infected cattle is based. We sampled 40 isolates from six countries widely distributed across the geographical range of the parasite, including eastern, central and southern Africa, for p104 sequence polymorphism. These included parasites from both domestic cattle and the Cape buffalo (Syncerus caffer) wildli

SUBMITTER: Obara I 

PROVIDER: S-EPMC10172223 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature