Immuno-PET Monitoring of CD8⁺ T Cell Infiltration Post ICOS Agonist Antibody Treatment Alone and in Combination with PD-1 Blocking Antibody Using a ⁸⁹Zr Anti-CD8⁺ Mouse Minibody in EMT6 Syngeneic Tumor Mouse.

ABSTRACT:

Purpose

The presence and functional competence of intratumoral CD8⁺ T cells is often a barometer for successful immunotherapeutic responses in cancer. Despite this understanding and the extensive number of clinical-stage immunotherapies focused on potentiation (co-stimulation) or rescue (checkpoint blockade) of CD8⁺ T cell antitumor activity, dynamic biomarker strategies are often lacking. To help fill this gap, immuno-PET nuclear imaging has emerged as a powerful tool for in vivo molecular imaging of antibody targeting. Here, we took advantage of immuno-PET imaging using ⁸⁹Zr-IAB42M1-14, anti-mouse CD8 minibody, to characterize CD8⁺ T-cell tumor infiltration dynamics following ICOS (inducible T-cell co-stimulator) agonist antibody treatm

SUBMITTER: Alsaid H 

PROVIDER: S-EPMC10172244 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature