Fim3-dependent autoagglutination of Bordetella pertussis.

ABSTRACT: Autoagglutination (Agg) of Bordetella pertussis is often observed in clinical laboratory. However, its causal factors and frequency in circulating strains are unknown. Repeated single colony isolation enabled us to detect an Agg^- mutant in the supernatant of an Agg⁺ strain of B. pertussis. Whole-genome sequencing and immunoblot analysis disclosed that the Agg^- mutant had a single C-deletion in its fim3 promoter region (Pfim3) which abolished Fim3 fimbriae production. A B. pertussis fim3-knock out mutant also lacked the Agg⁺ phenotype. Agg⁺ clinical isolates were detected a higher production of Fim3 than Fim3-producing Agg^- isolates. B. pertussis is known to harbor multiple Pfim3 poly(C) lengths within a single strain culture

SUBMITTER: Otsuka N 

PROVIDER: S-EPMC10172299 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature