FGFR2 mutations promote endometrial cancer progression through dual engagement of EGFR and Notch signalling pathways.

ABSTRACT:

Background

Mutations in the receptor tyrosine kinase gene fibroblast growth factor receptor 2 (FGFR2) occur at a high frequency in endometrial cancer (EC) and have been linked to advanced and recurrent disease. However, little is known about how these mutations drive carcinogenesis.

Methods

Differential transcriptomic analysis and two-step quantitative real-time PCR (qRT-PCR) assays were applied to identify genes differentially expressed in two cohorts of EC patients carrying mutations in the FGFR2 gene as well as in EC cells harbouring mutations in the FGFR2. Candidate genes and target signalling pathways were investigated by qRT-PCR assays, immunohistochemistry and bioinformatics analysis. The functional roles of differently regulated genes were analysed using in vitro and

SUBMITTER: Dixit G 

PROVIDER: S-EPMC10172618 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature