Effect of chronic intermittent ethanol vapor exposure on RNA content of brain-derived extracellular vesicles.

ABSTRACT: Extracellular vesicles (EVs) are important players in normal biological function and disease pathogenesis. Of the many biomolecules packaged into EVs, coding and noncoding RNA transcripts are of particular interest for their ability to significantly alter cellular and molecular processes. Here we investigate how chronic ethanol exposure impacts EV RNA cargo and the functional outcomes of these changes. Following chronic intermittent ethanol (CIE) vapor exposure, EVs were isolated from male and female C57BL/6J mouse brain. Total RNA from EVs was analyzed by lncRNA/mRNA microarray to survey changes in RNA cargo following vapor exposure. Differential expression analysis of microarray data revealed a number of lncRNA and mRNA types differentially expressed in CIE compared to control EVs. Weigh

SUBMITTER: Baratta AM 

PROVIDER: S-EPMC10173183 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature