Beet cyst nematode HsSNARE1 interacts with both AtSNAP2 and AtPR1 and promotes disease in Arabidopsis.

ABSTRACT:

Introduction

Plant parasitic cyst nematodes secrete a number of effectors into hosts to initiate formation of syncytia and infection causing huge yield losses.

Objectives

The identified cyst nematode effectors are still limited, and the cyst nematode effectors-involved interaction mechanisms between cyst nematodes and plants remain largely unknown.

Methods

The t-SNARE domain-containing effector in beet cyst nematode (BCN) was identified by In situ hybridization and immunohistochemistry analyses. The mutant of effector gene was designed by protein structure modeling analysis. The functions of effector gene and its mutant were analyzed by genetic transformation in Arabidopsis and infection by BCN. The protein-protein interaction was analyzed by yeast two hybrid, BiFC an

SUBMITTER: Zhao J 

PROVIDER: S-EPMC10173200 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature