New Approach for the Identification of Isobaric and Isomeric Metabolites.

ABSTRACT: The structural elucidation of metabolite molecules is important in many branches of the life sciences. However, the isomeric and isobaric complexity of metabolites makes their identification extremely challenging, and analytical standards are often required to confirm the presence of a particular compound in a sample. We present here an approach to overcome these challenges using high-resolution ion mobility spectrometry in combination with cryogenic vibrational spectroscopy for the rapid separation and identification of metabolite isomers and isobars. Ion mobility can separate isomeric metabolites in tens of milliseconds, and cryogenic IR spectroscopy provides highly structured IR fingerprints for unambiguous molecular identification. Moreover, our approach allows one to identify metaboli

SUBMITTER: Ben Faleh A 

PROVIDER: S-EPMC10173252 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature