Flux Sampling in Genome-scale Metabolic Modeling of Microbial Communities.

ABSTRACT: Microbial communities play a crucial role in ecosystem function through metabolic interactions. Genome-scale modeling is a promising method to understand these interactions. Flux balance analysis (FBA) is most often used to predict the flux through all reactions in a genome-scale model. However, the fluxes predicted by FBA depend on a user-defined cellular objective. Flux sampling is an alternative to FBA, as it provides the range of fluxes possible within a microbial community. Furthermore, flux sampling may capture additional heterogeneity across cells, especially when cells exhibit sub-maximal growth rates. In this study, we simulate the metabolism of microbial communities and compare the metabolic characteristics found with FBA and flux sampling. We find significant differences in the

SUBMITTER: Gelbach PE 

PROVIDER: S-EPMC10173371 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature