PIK3CA mutation in endometriotic epithelial cells promotes viperin-dependent inflammatory response to insulin.

ABSTRACT: Endometrial epithelia are known to harbor cancer driver mutations in the absence of any pathologies, including mutations in PIK3CA. Insulin plays an important role in regulating uterine metabolism during pregnancy, and hyperinsulinemia is associated with conditions impacting fertility. Hyperinsulinemia also promotes cancer, but the direct action of insulin on mutated endometrial epithelial cells is unknown. Here, we treated 12Z endometriotic epithelial cells carrying the PIK3CA^H1047R oncogene with insulin and examined transcriptomes by RNA-seq. While cells naively responded to insulin, the magnitude of differential gene expression (DGE) was nine times greater in PIK3CA^H1047R cells, representing a synergistic effect between insulin signaling and PIK3CA^H1047R

SUBMITTER: Wilson MR 

PROVIDER: S-EPMC10173629 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature