Dual roles of R-loops in the formation and processing of programmed DNA double-strand breaks during meiosis.

ABSTRACT:

Background

Meiotic recombination is initiated by Spo11-dependent programmed DNA double-strand breaks (DSBs) that are preferentially concentrated within genomic regions called hotspots; however, the factor(s) that specify the positions of meiotic DSB hotspots remain unclear.

Results

Here, we examined the frequency and distribution of R-loops, a type of functional chromatin structure comprising single-stranded DNA and a DNA:RNA hybrid, during budding yeast meiosis and found that the R-loops were changed dramatically throughout meiosis. We detected the formation of multiple de novo R-loops in the pachytene stage and found that these R-loops were associated with meiotic recombination during yeast meiosis. We show that transcription-replication head-on collisions could promote R-

SUBMITTER: Liu C 

PROVIDER: S-EPMC10173651 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature