HSPB7 oppositely regulates human mesenchymal stromal cell-derived osteogenesis and adipogenesis.

ABSTRACT:

Background

Recent evidence suggests that accumulation of marrow adipose tissue induced by aberrant lineage allocation of bone marrow-derived mesenchymal stromal cells (BMSCs) contributes to the pathophysiologic processes of osteoporosis. Although master regulators of lineage commitment have been well documented, molecular switches between osteogenesis and adipogenesis are largely unknown.

Methods

HSPB7 gene expression during osteogenic and adipogenic differentiation of BMSCs was evaluated by qPCR and Western blot analyses. Lentiviral-mediated knockdown or overexpression of HSPB7 and its deletion constructs were used to assess its function. The organization of cytoskeleton was examined by immunofluorescent staining. ALP activity, calcium assay, Alizarin Red S staining and Oil

SUBMITTER: Zhang S 

PROVIDER: S-EPMC10173662 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature