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Genetic characterization of human echinococcosis in Southern Punjab, Pakistan.


ABSTRACT:

Introduction

Echinococcosis is a neglected tropical zoonotic infection that affects both the human and livestock populations. In Pakistan, the infection is long-standing, but data on its molecular epidemiology and genotypic characterization in the southern Punjab region are limited. The aim of the current study was the molecular characterization of human echinococcosis in southern Punjab, Pakistan.

Methods

Echinococcal cysts were obtained from a total of 28 surgically treated patients. Patients' demographic characteristics were also recorded. The cyst samples were subjected to further processing to isolate DNA in order to probe the Nad1 and Cyt-b genes, followed by DNA sequencing and phylogenetic analysis for genotypic identification.

Results

The majorit

SUBMITTER: Basharat N 

PROVIDER: S-EPMC10174045 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature