Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer.

ABSTRACT:

Background

Nuclear Yes1-associated transcriptional regulator (YAP1) promotes tumor progression. However, the function of cytoplasmic YAP1 in breast cancer cells and its impact on the survival of breast cancer patients remain unclear. Our research aimed to explore the biological function of cytoplasmic YAP1 in breast cancer cells and the possibility of cytoplasmic YAP1 as a predictive marker of breast cancer survival.

Methods

We constructed cell mutant models, including NLS-YAP1^5SA (nuclear localized), YAP1^S94A (incapable of binding to the TEA domain transcription factor family) and YAP1^S127D (cytoplasmic localized), and used Cell Counting Kit-8 (CCK-8) assays, 5-ethynyl-2'-deoxyuridine (EdU) incorporation assays, and Western blotting (WB) ana

SUBMITTER: Guo Y 

PROVIDER: S-EPMC10174091 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature