The interplay of intracellular calcium and zinc ions in response to electric field stimulation in primary rat cortical neurons in vitro.

ABSTRACT: Recent pharmacological studies demonstrate a role for zinc (Zn²⁺) in shaping intracellular calcium (Ca²⁺) dynamics and vice versa in excitable cells including neurons and cardiomyocytes. Herein, we sought to examine the dynamic of intracellular release of Ca²⁺ and Zn²⁺ upon modifying excitability of primary rat cortical neurons using electric field stimulation (EFS) in vitro. We show that exposure to EFS with an intensity of 7.69 V/cm induces transient membrane hyperpolarization together with transient elevations in the cytosolic levels of Ca²⁺ and Zn²⁺ ions. The EFS-induced hyperpolarization was inhibited by prior treatment of cells with the K⁺ channel opener diazoxide. Chemical hyperpolarization had no ap

SUBMITTER: Alshawaf AJ 

PROVIDER: S-EPMC10174245 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature