Clinical relevance of cell-free DNA quantification and qualification during the first month after lung transplantation.

ABSTRACT:

Background

Many studies have reported the relevance of donor-derived cfDNA (dd-cfDNA) after lung transplantation (LTx) to diagnose and monitor acute rejection (AR) or chronic rejection or infection (INF). However, the analysis of cfDNA fragment size has not been studied. The aim of this study was to determine the clinical relevance of dd-cfDNA and cfDNA size profiles in events (AR and INF) during the first month after LTx.

Methods

This prospective, single-center study includes 62 LTx recipients at the Marseille Nord Hospital, France. Total cfDNA quantification was performed by fluorimetry and digital PCR, dd-cfDNA by NGS (AlloSeq cfDNA-CareDX^®), and the size profile by BIABooster (Adelis^®). A bronchoalveolar lavage and transbronchial biopsies at D30 est

SUBMITTER: Pedini P 

PROVIDER: S-EPMC10174290 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature