Comparison of the effects of oxidative and inflammatory stresses on rat chondrocyte senescence.

ABSTRACT: Osteoarthritis (OA) is an age-related degenerative joint disease that causes progressive cartilage loss. Chondrocyte senescence is a fundamental mechanism that contributes to the imbalance of matrix homeostasis in OA by inducing senescence-associated secretory phenotype (SASP). Although OA chondrocytes are mainly exposed to oxidative and inflammatory stresses, the role of these individual stresses in chondrocyte senescence remains unclear. In this study, we compared the effects of these stresses on the senescence of rat chondrocytes. Rat chondrocytes were treated with H₂O₂ and a combination of IL-1β and TNF-α (IL/TNF) to compare their in vitro effect on senescent phenotypes. For in vivo evaluation, H₂O₂ and IL/TNF were injected into rat knee join

SUBMITTER: Yagi M 

PROVIDER: S-EPMC10175275 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature