Transcriptome analysis of ovules offers early developmental clues after fertilization in Cicer arietinum L.

ABSTRACT: Chickpea (Cicer arietinum L.) seeds are valued for their nutritional scores and limited information on the molecular mechanisms of chickpea fertilization and seed development is available. In the current work, comparative transcriptome analysis was performed on two different stages of chickpea ovules (pre- and post-fertilization) to identify key regulatory transcripts. Two-staged transcriptome sequencing was generated and over 208 million reads were mapped to quantify transcript abundance during fertilization events. Mapping to the reference genome showed that the majority (92.88%) of high-quality Illumina reads were aligned to the chickpea genome. Reference-guided genome and transcriptome assembly yielded a total of 28,783 genes. Of these, 3399 genes were differentially expressed a

SUBMITTER: Singh R 

PROVIDER: S-EPMC10175530 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature