Development of a high-resolution mass-spectrometry-based method and software for human leukocyte antigen typing.

ABSTRACT:

Introduction

The human leukocyte antigen (HLA) system plays a critical role in the human immune system and is strongly associated with immune recognition and rejection in organ transplantation. HLA typing method has been extensively studied to increase the success rates of clinical organ transplantation. However, while polymerase chain reaction sequence-based typing (PCR-SBT) remains the gold standard, cis/trans ambiguity and nucleotide sequencing signal overlay during heterozygous typing present a problem. The high cost and low processing speed of Next Generation Sequencing (NGS) also render this approach inadequate for HLA typing.

Methods and materials

To address these limitations of the current HLA typing methods, we developed a novel typing technology based on nucleic ac

SUBMITTER: Wang K 

PROVIDER: S-EPMC10175642 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature