Rapid detection of Heterobasidion annosum using a loop-mediated isothermal amplification assay.

ABSTRACT: Heterobasidion annosum is one of the most aggressive pathogens of Pinus forests in Europe, causing considerable economic losses. To detect H. annosum for disease diagnosis and control, we developed a loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) DNA sequences of H. annosum. In our study, this LAMP assay was found to be capable of efficiently amplifying the target gene within 60 min at 63°C. In specificity tests, H. annosum was positively detected, and other species were negative. The detection limit of this assay was found to be 100 pg·μL^-1, and the assay was also successfully tested for use with basidiospore suspensions and wood samples. This study pr

SUBMITTER: Hong-Min Z 

PROVIDER: S-EPMC10175688 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature