MICU1 occludes the mitochondrial calcium uniporter in divalent-free conditions.

ABSTRACT: Mitochondrial Ca²⁺ uptake is mediated by the mitochondrial uniporter complex (mtCU) that includes a tetramer of the pore-forming subunit, MCU, a scaffold protein, EMRE, and the EF-hand regulatory subunit, MICU1 either homodimerized or heterodimerized with MICU2/3. MICU1 has been proposed to regulate Ca²⁺ uptake via the mtCU by physically occluding the pore and preventing Ca²⁺ flux at resting cytoplasmic [Ca²⁺] (free calcium concentration) and to increase Ca²⁺ flux at high [Ca²⁺] due to cooperative activation of MICUs EF-hands. However, mtCU and MICU1 functioning when its EF-hands are unoccupied by Ca²⁺ is poorly studied due to technical limitations. To overcome this barrier, we have studied the mtCU in divalent-fr

SUBMITTER: Rodriguez-Prados M 

PROVIDER: S-EPMC10175726 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature