High sensitivity top-down proteomics captures single muscle cell heterogeneity in large proteoforms.

ABSTRACT: Single-cell proteomics has emerged as a powerful method to characterize cellular phenotypic heterogeneity and the cell-specific functional networks underlying biological processes. However, significant challenges remain in single-cell proteomics for the analysis of proteoforms arising from genetic mutations, alternative splicing, and post-translational modifications. Herein, we have developed a highly sensitive functionally integrated top-down proteomics method for the comprehensive analysis of proteoforms from single cells. We applied this method to single muscle fibers (SMFs) to resolve their heterogeneous functional and proteomic properties at the single-cell level. Notably, we have detected single-cell heterogeneity in large proteoforms (>200 kDa) from the SMFs. Using SMFs obtained fro

SUBMITTER: Melby JA 

PROVIDER: S-EPMC10175728 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature