Single-nucleus and Spatially Resolved Intratumor Subtype Heterogeneity in Bladder Cancer.

ABSTRACT:

Background

Current bulk transcriptomic classification systems for bladder cancer do not consider the level of intratumor subtype heterogeneity.

Objective

To investigate the extent and possible clinical impact of intratumor subtype heterogeneity across early and more advanced stages of bladder cancer.

Design setting and participants

We performed single-nucleus RNA sequencing (RNA-seq) of 48 bladder tumors and additional spatial transcriptomics for four of these tumors. Total bulk RNA-seq and spatial proteomics data were available from the same tumors for comparison, along with detailed clinical follow-up of the patients.

Outcome measurements and statistical analysis

The primary outcome was progression-free survival for non-muscle-invasive bladder cancer. Cox reg

SUBMITTER: Lindskrog SV 

PROVIDER: S-EPMC10175738 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature