Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling network.

ABSTRACT: The epidermal growth factor receptor (EGFR) has been studied extensively because of its critical role in cellular signaling and association with disease. Previous models have elucidated interactions between EGFR and downstream adaptor proteins or showed phenotypes affected by EGFR. However, the link between specific EGFR phosphorylation sites and phenotypic outcomes is still poorly understood. Here, we employed a suite of isogenic cell lines expressing site-specific mutations at each of the EGFR C-terminal phosphorylation sites to interrogate their role in the signaling network and cell biological response to stimulation. Our results demonstrate the resilience of the EGFR network, which was largely similar even in the context of multiple Y-to-F mutations in the EGFR C-terminal tail, while

SUBMITTER: Gerritsen JS 

PROVIDER: S-EPMC10176108 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature