Evaluation of microRNA let-7b-3p expression levels in methamphetamine abuse.

ABSTRACT:

Objective

In this study, we aimed to identify a microRNA expression signature that could be used to distinguish methamphetamine from control samples. We also utilized the existing bioinformatics tools in order to predict the candidate microRNAs that could play potential key roles in regulating drug addiction-related genes.

Methods

Methamphetamine samples from 21 ventral tegmental area and 21 nucleus accumbens samples and their control regions were obtained from the Council of Forensic Medicine (Istanbul). Quantitative analysis of let-7b-3p was studied using quantitative reverse transcription PCR. Statistical analysis was carried out using Student's t-test. The receiver operating characteristic curves were plotted with Statistical Package for the Social Sciences (SPSS 20.0).<

SUBMITTER: Demirel G 

PROVIDER: S-EPMC10176632 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature